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β-Galactosidases catalyze the hydrolysis of β(1-3) and β(1-4) galactosyl bonds in oligosaccharides as well as the inverse reaction of enzymatic condensation and transglycosylation. Here we report the crystallographic structures of Penicillium sp. β-galactosidase and its complex with galactose solved by the SIRAS quick cryo-soaking technique at 1.90Å and 2.10Å resolution, respectively. The amino acid...
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