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The analytical procedure of thiamine quantification in premixtures and compound feedingstuffs by HPLC method is presented. Thiamine was extracted from feed samples with 0.1M hydrochloric acid at 100°C for 30 min. In the case of compound feedingstuffs, 10% Taka-diastase solution was added to the samples, and then the samples were incubated at 37°C for 17 h. Afterwards, thiamine was oxidised to thiochrom,...
The paper presents results from the comparative studies upon methods of industrial fodder mixtures homogeneity evaluation. Competence of participating laboratories to make a legal control within that issue was confirmed. Achieved results on the mixing level (variability coefficient) of fodder mixtures for turkeys and swine were lower than 10% – limit value. Reproducibility limits of results achieved...
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