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The current paper reports on an investigation of the kinetics of chitosan deacetylation by chitin deacetylase isolated from Absidia orchidis vel coerulea. The reaction rate was correlated with the concentration of GlcNHAc units of the polymer. It is shown that the process follows the Michaelis–Menten mechanism. Modification of the Michaelis–Menten equation by introducing the activity of the enzyme...
In the paper the thermal deactivation of chitin deacetylase separated from Absidia orchidis vel coerulea was investigated. Experiments were carried out at a temperature of 50ºC up to 24 hours. Three mechanisms of deactivation were tested: irreversible first-order, reversible firstorder, and three-parameters phenomenological model. It was found that the last one gave the best approximation of the deactivation...
Opisano sposoby hodowli grzybów strzępkowych Absidia orchidis wykorzystywanych do produkcji chitozanu pochodzenia mikrobiologicznego. Największą wydajność chitozanu uzyskiwano w powtarzanych hodowlach okresowych. W długoterminowych hodowlach obserwowano zmianę morfologii grzybów z form strzępkowych w drożdże absidiowe. Wyznaczono i porównano właściwe szybkości wzrostu dla form strzępkowych (ls= 0,122...
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