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The hydrolysis of urea in phosphate buffer (pH 7.0) at 25°C in the presence of sodium fluoride as an urease inhibitor was studied. It was found that fluoride ion is a competitive slow-binding inhibitor of the enzyme and the constants of inhibition were measured.The progress of the reaction was observed by means of a classical analytical method (increase of NH 3 concentration) and a calorimetric...
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