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Recently, numerous plant-based preparations have been used for health promotion or disease prevention in animals. Chicory is one of the plants that contain various nutraceutics, mainly inulin type fructans (ITFs) showing prebiotic character. In pigs, a significant proportion of ITFs is fermented in the distal part of the small intestine to lactate and short-chain fatty acids (SCFAs). Previous studies...
The analysis of mucosa proteomes was performed using a twodimensional electrophoresis combined with mass spectrometry to determine the effect of dietary level of inulin on protein expression patterns in the ileum. The experiment was carried out on 24 castrated male piglets, allocated to three groups, fed from the day 10 of life an unsupplemented diet (C) or diet supplemented with 1% (T1) or 3% (T2)...
The aim of the study was to estimate the influence of dietary supplementation with dried chicory root and native chicory inulin on biochemical profile of blood plasma. In total 24 male piglets were divided into 3 groups (n = 8) and fed unsupplemented cereal-based diet (C diet), diet with 2% of native chicory inulin (T1 diet) or with 4% of dried chicory root (T2 diet). After 40 days of feeding pigs...
The aim of the study was to determine whether non-essential amino acids (NEAA) in form of wheat gluten (WG) have a threonine (Thr)- sparing effect on gut structure in young pigs. A two-factorial experiment was conducted on 12 groups of 6 pigs. Pigs of 12.5 kg initial body weight (BW) were fed for about 20 days diets differing in the Thr level (5.1, 5.7, 6.3 and 6.9 g standardized ileal digestible...
In an in vitro experiment with pig inoculum, the main and interactive effects of type of protein and fibre on the concentrations of short-chain fatty acids (SCFA) and amines as the end products of fermentation and proteolysis were determined. The effects of inoculation with colonic digesta (C) sampled post mortem or with freshly voided faeces (F) as the sources of microflora, and of 24- vs 48-h periods...
The effects of genetically modified (GM) soyabean meal (SBM) and GM maize on the functional status of the small intestine of broiler chickens was studied. Eight diets were prepared, based on conventional or GM SBM (GTS 40-3-2) combined with non-modified maize cvs. Clarica and PR39 F58 or their isogenic GM (MON 810) counterparts cvs. Bacilla or PR39 F56. Diets were fed in mash form from days 1 to 30...
Autoclaving is the current method of choice for sterilization of diets for specified-pathogen-free (SPF) laboratory animals, but this process may negatively affect the nutrient content (e.g., protein, vitamin) of the diets. Two natural-ingredient diets supplemented with either soyabean meal containing phytoestrogens (S) or with casein (C), and a commercial autoclavable (SN) diet, were autoclaved at...
The high price of pure forms of solanidine glycoalkaloids (GA) impedes their use in studies on the effects and safety levels of GA for farm animals. An attempt was made to take advantage of the high concentration of GA in potato sprouts and to evaluate them as a potential source of these compounds for nutritional experiments. Over a period of two years, two batches of sprouts were collected from potatoes...
The effects of potato protein concentrates (PPC) differing in dietary solanidine glycoalkaloids (SGA) and trypsin inhibitor activity (TI), and of potato sprouts with differing SGA levels, were studied in rats. In Experiment 1, semi-purified isoprotein diets containing casein or one of six PPC contributing from 116 to 439 mg SGA · kg–1 and from 0.13 to 0.45 mg · g–1 of TI activity, were used. Weight...
The development of the gastrointestinal tract is a very sophisticated process, which starts during prenatal life and continues after birth. Diet is the most important factor modulating structure and functions of the intestine. In the early post-natal period the major role play bioactive substances of colostrum and milk. Their properties influence the dynamics of apoptosis and mitosis and through gastrointestinal...
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