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In the preceding research, stevia has been typically cloned in vitro using two media, on which the shoots were formed (3–6 weeks), and on the other they were rooted (3–5 weeks). This study aimed at finding the possibility for rapid stevia propagation from large nodal explants using the MS basal medium [Murashige and Skoog 1962], with low auxin concentrations (0.5, 1 and 2 mg.dm–3). The plants were...
Wierzchołki pędów o długości 5-10 mm, wycięte ze sterylnych siewek dwóch odmian koniczyny białej (Romena i Wota), wyłożono do szalek Petriego na 12 pożywkach indukcyjnych. Zawierały one składniki zmodyfikowanej pożywki MS (z witaminami pożywki B5 i czterokrotnym stężeniem mikroelementów) i różniły się stężeniem hormonów (od 0 do 0,6 mg∙dm-3 NAA i od 0 do 6 mg∙dm-3 BAP). Szalki umieszczono na półkach...
Z liści 50 roślin mniszka lekarskiego wycięto eksplantaty (0,5 cm2), i po odkażeniu w 0,1% HgCl2 (2 minuty) i wyłożono do szalek Petriego na 10 pożywek. Stosowano pożywkę podstawową MS, kombinacje doświadczalne różniły się zawartością kinetyny (od 0,5 do 1,5 mg∙dm-3 KT) i kwasu indolilo-3-octowego (od 0 do 0,3 mg∙dm-3 IAA). Szalki wyłożono na półkach pokoju hodowlanego. Po pięciu tygodniach policzono...
Ten Polish red clover cultivars were tested in vitro for their capability to produce callus and somatic embryos. A three-step tissue culture protocol (callus induction, embryo induction and plant development) based on Gamborg's B5 basal salts, NAA and 2,4-D as auxins, and kinetin and adenine as cytokinins was utilized. Explants source were petiole and hypocotyl sections. All clover varieties under...
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