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The aim of this study was the determination of the occurence of ICP4, pp38, meq, and LAT genes of Marek's disease virus (MDV) and its rate of replication in internal organs of infected chickens. The genes were determined using PCR, while the virus replication was measured by real-time PCR method. The results have shown the presence of ICP4 and pp38 genes starting from the 3rd d p.i. while meq oncogene...
To increase the diagnostic capacity of PCR, duplex PCR for the detection of the Meq gene and 132 bp sequence of Marek's disease virus (MDV), serotype 1 has been developed. The reaction enabled the differentiation of vaccine and field strains of MDV among serotype 1 of the virus. Additionally, it gave preliminary information about eventual vaccination with the Rispens strain.
The aim of this study was an attempt to apply PCR for diagnosis of Marek's disease. Eighteen field Marek's disease virus strains and 4 standard strains: virulent HPRS₁₆ strain and attenuated CVI 988 strain (serotype 1 - MDV-1), apathogenic SB 1strain (serotype 2 - MDV-2) and non - pathogenic turkeys HVT FC 126 strain (serotype 3 MDV-3) were used. Chicken anaemia virus (CAV) and infectious laryngotracheitis...
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