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The aim of this study was the determination of the occurence of ICP4, pp38, meq, and LAT genes of Marek's disease virus (MDV) and its rate of replication in internal organs of infected chickens. The genes were determined using PCR, while the virus replication was measured by real-time PCR method. The results have shown the presence of ICP4 and pp38 genes starting from the 3rd d p.i. while meq oncogene...
The aim of this study was to compare a standard polymerase chain reaction (PCR) with real-time PCR for the detection of meq gene for the Marek's disease virus. Forty-one samples were examined in the experiment. In the standard PCR, the product characteristic for field strains (1 062 bp) was obtained in 19 samples (positive samples), in 22 samples no bands on the gel were observed (negative samples)...
To increase the diagnostic capacity of PCR, duplex PCR for the detection of the Meq gene and 132 bp sequence of Marek's disease virus (MDV), serotype 1 has been developed. The reaction enabled the differentiation of vaccine and field strains of MDV among serotype 1 of the virus. Additionally, it gave preliminary information about eventual vaccination with the Rispens strain.
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