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A human lactoferrin-specific cell line was generated in CBA mice, sensitized with 200 μg HLF in Freund’s complete adjuvant. HLFK1 cells derived from the lymph nodes of these mice were maintained using HLF as the antigen. HLF was added at the beginning of each 14-day restimulation cycle, at a concentration of 100 μg/ml. The presentation of the antigen to HLFK1 was demonstrated using glass-adherent...
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