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The aim of this study was to assess the inbreeding coefficient of Polish Konik horses based on runs of homozygosity (ROH). Ninety six horses kept in 6 herds located across Poland were genotyped with the use of EquineSNP60 BeadChip (Illumina). SNP markers with a Minor Allele Frequency lower than 0.01 and SNPs assigned to chromosome X or Y were excluded from the study. A total of 50 708 SNPs were included...
The aim of this study was to investigate the effect of PAF supplementation in semen extender on ATP content in cryopreserved bull spermatozoa used for artificial insemination at different time intervals. Cryopreserved semen was treated with different concentrations of PAF: 1x10⁻⁵M, 1x10⁻⁶M, 1x10⁻⁷M, 1x10⁻⁸M and 1x10⁻⁹M at 37°C. In the present work we showed that content of ATP in cryopreserved semen...
The aim of the study was to find out whether carriers of new genetic defect Cholesterol Deficiency (CD) occur in the population of Polish Holstein-Friesian bulls. Twenty seven bulls were included in the analysis. Bulls were selected as having in the pedigree known carrier of CD (Maughlin Storm CANM000005457798). All bulls were diagnosed by the test described by Menzi et al. (2016) by using allele-specific...
The aim of this study was to evaluate the effect of C/T missense mutation (SOX5) gene on selected parameters of non-frozen semen in Polish Holstein – Friesian bulls. Three hundred and sixty eight bulls were genotyped by the PCR-Rsa I technique. Semen was collected and evaluated by standardized procedure used in an AI station. Significant associations were found between SOX5 genotypes and sperm concentrations...
The aim of the study was to determine the effects of platelet-activating factor (PAF) on selected quality parameters of cryopreserved bull semen with reduced sperm motility used for artificial insemination. The aim of experiment 1 was to identify the optimal concentration of the phospholipid able to preserve sperm viability. Cryopreserved semen was treated with different PAF concentrations: 1x10-5M,...
The aim of this paper was to verify the hypothesis whether carriers of genetic defect Brachyspina occur in the Polish Holstein-Friesian Cattle. PCR method was used to screen 78 Polish Hol-stein-Friesian bulls. Eight bulls were identified as heterozygotes for 3,3 kb deletion in the FANCI gene - the mutation causing Brachyspina defect. All carriers were sons of 3 sires: Cleitus Jabot, Sandy-Valley Bolton...
Selected qualitative and biochemical parameters were determined in cryopreserved semen used for artificial insemination, sampled from 120 bulls reared at the Animal Breeding and Insemination Center in Bydgoszcz. The total average motility of the analyzed sperm samples was determined at 62.51%. The percentage of motile spermatozoa displaying progressive forward motility was 21.65%. Analyzed samples...
This study analyzed the association between single nucleotide polymorphism (A/C) in position -28 located in the TATA box of LTF gene and the lactoferrin concentration in bovine milk secreted by healthy and infected udders. Out of 241, 69 cows were selected into the experimental group and were divided into 3 groups according to mean value of somatic cell count (SCC): I <180 000 cells/mL, II: 180...
The aim of the study was to find out whether the single nucleotide polymorphism (SNP) within arylsulfatase D (ARSD) gene is associated with kinematic parameters of sperm motility in Holstein- Friesian bulls. 367 Holstein-Friesian bulls kept in one AI center were included in the study. Point mutation C/T at position 139037255 on chromosome X (rs42207167) was identified by PCR-RFLP method (Pflm I)....
The effectiveness of a program aimed at eradicating carriers of the recessive disorder Complex Vertebral Malformation (CVM) from the population of Holstein-Friesian bulls is reported. Among 1823 bulls, 1268 young and 555 proven bulls were examined. Three hundred and three bulls appeared to be CVM carriers (16.62%). The highest number of carriers occurred in the sons of a CVM sire, 55.51% and 61.90%,...
Modern pig production needs new tools for fast, reliable, more effective breeding. In the present paper we present a chip containing 45 SNP (Single Nucleotide Polymorphisms) which enables the determining of 1 genetic disease (PSS – Porcine Stress Syndrome), 4 QTLs genes: PRKAG3, CAST, MC4R and ESR, which together with the remaining SNPs create a panel useful in marker-assisted selection and veterinary...
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