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An innovatory protocol for large-scale production of narcissus ‘Carlton’ somatic embryos was developed based on the repetitive somatic embryogenesis (RSE). RSE was established as a stepwise process beginning with primary somatic embryogenesis (PSE) on ovary explants followed by secondary somatic embryogenesis (SSE) and continuously repeating cycles of SSE. A highly embryogenic lines of callus were...
The effect of the concentration and proportion of growth regulators in an initial medium on Narcissus L. ‘Carlton’ morphogenesis in cultures of Pc stage flower bud ex-plants was investigated. Ovary and flower stem explants were cultured on solid Mura-shige and Skoog (MS) media with cytokinin – 6-Benzyladenine (BA) or Zeatin (ZEA) (0.5–50 μM) – in combination with auxin – 4-Amino-3,5,6-trichloropicolinic...
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