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In this short review I will first concisely describe the principles of single-molecule force spectroscopy (SMFS) for measuring the mechanical properties of individual polymeric molecules, as implemented on an Atomic Force Microscope (AFM) platform. Next, I will review a selected number of the most striking, in my opinion, discoveries and observations accumulated in this field of research that now...
In Escherichia coli, errors in newly-replicated DNA, such as the incorporation of a nucleotide with a mis-paired base or an accidental insertion or deletion of nucleotides, are corrected by a methyl-directed mismatch repair (MMR) pathway. While the enzymology of MMR has long been established, many fundamental aspects of its mechanisms remain elusive, such as the structures, compositions, and orientations...
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