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An engineered Pichia pastoris GS115 with a FIP‐glu gene was mutated using ultraviolet (UV) radiation, and a high‐throughput screening method was established for screening of high‐yield strains. Meanwhile, a preliminary study was conducted to determine the bioactivity of the rFIP‐glu. Based on OD600 value and the mortality of engineered P. pastoris GS115, the best UV irradiation time was determined...