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For the last few years, in the Department of Biology and Pharmaceutical Botany ? Plantago asiatica L. ? a medicinal plant, popular in the Far East, has been cultured in vitro. Two lines of the nonmorphogenic callus were obtained: cream- and green- coloured. They were induced from the roots taken from 4-week-old seedlings. The cream-coloured callus was cultured on Murashige and Skoog medium enriched...
2 Xylogenesis was observed in the morphogenic and nonmorphogenic calluses. The light microscopy was used for a histological study. The ultrastructure of the calluses was analysed using the JEM 100 B transmission electron microscope. Tracheary elements were formed in all studied kinds of calluses. They had lignified spiral or reticulate thickening of cell walls. In nonmorphogenic calluses, tracheary...
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