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The aim of this study was to determine the prevalence of bluetongue virus (BTV) in the blood of susceptible animals, tested in the frame of the BT national monitoring programme. The rRT-PCR assay was applied to virological examination of animals imported from BT-affected countries. On December 5, 2007, the BTV RNA was detected for the first time in blood samples of seropositive cattle from Germany...
Real-time RT-PCR (rRT-PCR) for the detection of bluetongue virus (BTV) in EDTA treated blood samples taken from BTV infected animals was described. A combination of two primer sets (representing eastern and western BTV serotypes) and two Taqman probes specific for a highly conserved region in BTV RNA segment 1 were used. The assay detected the viral RNA in blood samples collected from seropositive...
RT-PCR for the detection of bluetongue virus (BTV) in blood samples, collected from /infected animals, were described . Two primer sets targeting genome segment 7 of BTV were selected. The full-length S7 cDNA (1156 bp) was amplified in all samples of EDTA blood taken from BTV infected animals. No viral RNA was detected in samples from uninfected animals and seropositive cattle of Dutch origin, imported...
A study on the seroprevalence of FMD antibodies in cattle in the „ring” vaccination zone was performed. Out of 550 samples of sera examined by LPB ELISA, 240 samples (44%) have been found positive to FMDV type A,O and/or C (ELISA titres > log 1.6). The highest Ievel of antibodies was found for FMDV type O₁BFS; about 10% of tested sera had titres > log 3.01. A half-life of maternal antibodies...
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