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This study investigates the effect of zinc on morphogenic and biochemical responses of Bacopa monniera (L.) Wettst and determines the maximum zinc level to which tolerant cultures could be raised. Incorporation of zinc sulfate (100–600 μM) in the maintenance medium, i.e., Murashige and Skoog’s (MS) medium (sucrose 2%) supplemented with naphthaleneacetic acid (0.2 mg l −1 ), benzylaminopurine...
Presence of copper sulphate (25-100 μM) in the medium, MS (sucrose 2%) supplemented with NAA (0.2 mg l -1 ), BAP (0.5 mg l -1 ) and glutamine (50 mg l -1 ) significantly enhanced the growth of regenerants of Bacopa monniera. Higher concentrations (200-600 μM), however, inhibited shoot and root growth for 16 weeks, although the regenerants resumed growth thereafter...
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