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The culture conditions for extracellular protease production by two bacterial strains, Bacillus licheniformis BF2 and Bacillus subtilis BH4 isolated from the foregut and hindgut regions, respectively, of bata (Labeo bata, Cyprinidae) were optimized to obtain maximum level of protease. Both strains were cultured in TSA broth for 24 h at 37 ± 2 °C. This was used as the inoculum for the production medium...
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