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Diagnostics of Mycoplasma synoviae (MS) is generally based on serological, culturing and molecular methods. Rapid diagnosis and identification of infections is very important in the poultry industry. The use of PCR or real-time PCR makes it possible to shorten the time for obtaining results from research and the effective detection of genetic material of MS. There are many variations of the PCR method,...
The objective of the studies was development and use of a customized ELISA kit to diagnose mycoplasmal infections in poultry. The frozen strain of S-6 M. gallisepticum, concentrated and ultra-sounds processed, served as the antigen. The best extinction (OD) was noted with the antigen concentration of 100 µg/ml, sera diluted at 1:100 and conjugate diluted at 1:250. It was best to preserve antigen coated...
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