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Eukaryotic protein synthesis begins with assembly of 48S initiation complexes at the initiation codon of mRNA, which requires at least seven initiation factors (eIFs). First, 43S preinitiation complexes comprising 40S ribosomal subunits, eIFs 3, 2, 1, and 1A, and tRNA Meti attach to the 5′-proximal region of mRNA and then scan along the 5′ untranslated region (5′UTR) to the initiation...
Transcript-specific translational control restricts macrophage inflammatory gene expression. The proinflammatory cytokine interferon-γ induces phosphorylation of ribosomal protein L13a and translocation from the 60S ribosomal subunit to the interferon-γ-activated inhibitor of translation (GAIT) complex. This complex binds the 3′UTR of ceruloplasmin mRNA and blocks its translation. Here, we elucidate...
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