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Nanoflow liquid chromatography–electrospray ionization tandem–mass spectrometry (nanoLC–ESI–MS–MS) was applied for the characterization of intact phosphatidylcholine (PC) lipid molecules using a homemade reversed phase capillary column with a pulled tip for direct ESI at positive ion mode. Prior to the analytical column, a short capillary trapping column was utilized for on-line pre-concentration...
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