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Disease resistance gene cloning is one of the main targets in modern sugarcane research. In this study, several primers were designed according to the conserved motifs in the NBS regions of known resistance genes to amplify sequences from cDNA of sugarcane variety NCo376 by polymerase chain reaction (PCR). In total, six NBS-LRR type resistance gene analogs (RGAs) were cloned, for which the deduced...
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