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It was found that the hydrolysis of 9-(4-chlorophenyloxycarbonyl)-10-methylacridinium triflate (CPOCMA) could be catalyzed by recombinant human PON1. Based on this property, the CPOCMA was evaluated as a substrate for serum PON activity assay. The apparent K m value of a serum sample for the substrate was determined as 85nmol/L, close to the K m value (83nmol/L) of rHuPON1. The interferences...
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