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Background & Aims: We report a novel approach to study biliary water, bile acid, and HCO3− transport: the microperfusion of intrahepatic bile duct units (IBDUs) isolated from normal rat liver. Methods: To study water transport, IBDUs were perfused in vitro with a membrane-impermeant fluorescent volume marker, fluorescein sulfonate; net water movement (Jv) and osmotic water permeability (Pf) were...
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