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In this paper, we developed a simple, ultra-sensitive and label-free fluorescent probe for trypsin and its inhibitor based on DNA hosted Cu nanoclusters (Cu NCs). Fluorescent Cu nanoclusters with maximum emission wavelength of 591nm are formed by using double-stranded DNA (dsDNA) as templates. With the addition of protamine, the fluorescence intensity (FL) of Cu NCs could be enhanced obviously through...
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