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Metal‐ion‐responsive transcriptional regulators within the MerR family effectively discriminate between mono‐ and divalent metal ions. Herein we address the origin of the specificity of the CueR protein for monovalent metal ions. Several spectroscopic techniques were employed to study AgI, ZnII, and HgII binding to model systems encompassing the metal‐ion‐binding loop of CueR from E. coli and V. cholerae...
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