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A novel dual-competitive lateral flow aptasensor (LFA) benefited from aptamer and lateral flow strips was first designed by using aflatoxin B1 (AFB1) as the model target. In this LFA assay, the target AFB1 competed with AFB1-hapten at T line for binding to Cy5-labeled AFB1 aptamer and the complementary strand competed with the target AFB1 for binding to Cy5-labeled AFB1 aptamer at C line. The ratio...
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