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Rat liver microsomal cytochrome P-450 d was abundantly expressed in the yeast Saccharomyces cerevisiae by using a yeast-Escherichia coli shuttle vector consisting of rat liver P-450 d cDNA and yeast acid phosphatase promoter. The expressed cytochrome P-450 d was immunologically crossed with rat liver P-450 d . The hydroxylase activity of estra-1,3,5(10)-triene-3,17β-diol...
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