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Laserowa jonizacja próbki wspomagana matrycą (MALDI - Matrix Assisted Laser Desorption/Ionization) jest nową metodą stosowaną w spektrometrii masowej od polowy lat osiemdziesiątych w badaniach ciężkich biomolekuł. Wykorzystuje ona stalą matrycę, która pełni kluczową rolę w ich procesach jonizacyjnych. Procesy te (desorpcja, jonizacja) inicjuje tutaj krótkotrwały (kilka nanosekund) impuls światła laserowego...
Nowadays, the time of flight mass spectrometry (TOF MS) is developed intensively. Advantages of this technique are in practice unlimited range of registered masses and high resolution. These both give ability of detecting high mass of molecules and studying of ionization processes connected with them. The time of flight mass spectrometer constructed and used for MALDI (Matrix Assisted Laser Desorption...
MALDI (Matrix Assisted Laser Desorption/lonization is one of the most sensitive methods used for analysis of large molecules such as peptides, proteins and nucleotides. In the seventies plasma desorption, field desorption, chemical ionization, secondary ions emission and chemical ionization methods were used for studies on these molecules. They met many problems connected mainly with instability of...
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