The Infona portal uses cookies, i.e. strings of text saved by a browser on the user's device. The portal can access those files and use them to remember the user's data, such as their chosen settings (screen view, interface language, etc.), or their login data. By using the Infona portal the user accepts automatic saving and using this information for portal operation purposes. More information on the subject can be found in the Privacy Policy and Terms of Service. By closing this window the user confirms that they have read the information on cookie usage, and they accept the privacy policy and the way cookies are used by the portal. You can change the cookie settings in your browser.
This paper describes a core-shell hydrogel microfiber consisted of alginate and tetra-arm poly(ethylene glycol) (tetra-PEG) encapsulating rat insulinoma cells using a microfluidic process with a coaxial device and rapid gelation induced by diffusion of two different cross-linkers of barium ion and dithiothreitol (DTT) in the laminar flow (Fig. 1). Microfluidic techniques are able to encapsulate the...
This paper describes the construction and differentiation of human adipose-derived stem cell (ADSC) fibers into the adipocyte lineage for breast reconstruction. Human ADSCs were encapsulated into the core region of hydrogel core-shell fibers by the cell fiber technology [1], induced for adipogenic differentiation, and maintained for over 2 months. After adipogenic induction, accumulation of lipid...
We report a method to measure contractile forces of cardiomyocytes at cellular level using microplates; pairs of single-cell sized plates are connected by a flexible hinge at the center. Cardiomyocytes repeatedly contract and relax, and thereby fold and stretch the microplates at the flexible hinge. By measuring the change of the bending angle in folded microplates, we can estimate contractile forces...
We report the multiphoton fabrication of hybrid microstructures of photoresist and chemically responsive protein hydrogel for microactuation, such as a lever and a rotary stepper. By two-step direct laser writing (DLW) technology, we combine chemically responsive protein hydrogel with mechanical robust photoresist into pH-responsive hybrid actuators that contain only biocompatible materials. The fabrication...
Microenvironmental biophysical stimuli influence diverse cellular functions, such as directional motility and stem cell differentiation. Previously, researchers have tuned the linear stiffness of microposts to investigate cell mechanobiological processes and direct cellular behavior; however, microposts suffer from an inherent, yet critical drawback — regulation of micropost stiffness is fundamentally...
This paper describes preliminary result of a specially patterned and aligned neural bundle formed by neural stem cell (NSC) microfibers for neural transplantation. The NSC microfibers are made by core-shell hydrogel microfiber encapsulating mouse NSCs. We developed a technique to align microfibers in a straight line and arrange the fibers in special patterns. Using this technique, we formed a neural...
We present a cell handling method using magnetically functionalized microplates. Rectangular-shaped permalloy inside the microplates enables the plates to rotate or flip upside down by magnetic field. We conduct two kinds of cell handling experiments: (1) rotating cells for the precise positioning of cell nucleus and (2) flipping over the microplate for culturing different types of cells on both sides...
Set the date range to filter the displayed results. You can set a starting date, ending date or both. You can enter the dates manually or choose them from the calendar.