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This work presents a method to detect volatile odorant using corneal epithelial cells with olfactory receptors. Because the corneal epithelial cells can survive at constant exposure of air, these cells have ability to detect the volatile odorant at air-liquid phase in contrast to other parenchymal cells. Therefore, in the present study, we first fabricate a device that provides the air-liquid environment...
A rapid and high-resolution bioprinter based on a capillary coaxial microfluidic printer head and vacuum substrate, was conceived and built up in this work. We demonstrate the printing of porous (400 μm pitch) 3D bio-scaffolds made from calcium alginate microfibers with a printing resolution (i.e. fiber diameter) of ca. 150 microns, at a speed of 40 mm/s, which is approximately 10x faster than existing...
This study reports an efficient droplet interface bilayer (DIB) formation using an open chamber with an external separator that can divide an aqueous solution to two regions. This approach facilitates reagent-saving and simplification of the device fabrication. Amphiphobic modification of the separator surface enables us to handle the device easily without liquid spill, and assists bilayer lipid membrane...
This paper highlights the behavior of microdroplets used for portable biosensors. Microdroplets have been applied for various Lab-on-a-chip applications, in which portability/wearable is becoming a trend word. However, the resonance (sloshing phenomena) of the microdroplets could be an issue. For example, bilayer lipid membrane, fragile characteristics due to its thickness of 5 nm, easily ruptures...
We develop a device adapted for repetitive formation of horizontal lipid bilayer membranes. This device allows simultaneous optical and electrophysiological measurements of the formed membranes. We integrated a rotational chamber on a fluidic channel separated by a parylene micropore. The rotational motion is designed to form a bilayer repeatedly. This rotational process emulates the conventional...
Transmembrane proteins functioning as the cellular signaling gateway are the major molecular targets of commercial drug therapy. HEK293T cells are world widely used cell based-platform for these proteins analysis. We loaded the photo-curable hydrogel into the HEK293T cells, treated with UV through the micron photo-mask, and fabricated the cell membrane fiber. The activity of ion channels on the surface...
This paper describes a contactless manipulation system for giant liposomes in an aqueous flow by negative dielectrophoresis (nDEP). The system consists of 2 pairs of comb electrodes that face each other across a perpendicular channel. This design allows an nDEP force to be focused at the center of the gap of the electrodes and the liposomes to align in lines without contacting either the electrodes...
This paper describes the preparation of a cell-sized vesicle containing small vesicles from a two-planar lipid bilayer in a triple-well device using a pulsed jet flow. First, the size distribution of the cell-sized vesicles is estimated using a double-well device wherein a separator having a hole diameter of 100, 200, or 500 μm is mounted between the wells. Moreover, the small vesicles are successfully...
This paper describes microfluidic logic gates which use DNA and biological nanopores. Single-stranded DNA (ssDNA) can pass through αHL, a biological nanopore, incorporated in bilayer lipid membranes (BLMs), whereas double-stranded DNA (dsDNA) cannot. In this study, these passing and non-passing phenomena were applied as the binary system and logic gates. Two types of ssDNA were used as inputs, while...
This letter describes the integration of an electrical connection onto a lipid bilayer membrane microchip for the electrical detection/regulation of transmembrane phenomena together with fluorescence monitoring. Arrayed microchambers with a volume of 3 pL were fabricated within a poly(dimethyl siloxane) microfluidic device, and electrical access was achieved by designing channels adjacent to the chambers...
This paper describes a microfluidic device for a high through-put ion channel recordings, in which planar bilayer lipid membranes (BLMs) are spanning across a nanometer-sized poly(p-xylylene) (parylene) pore (Figure 1). To make the nanopore, a micrometer-sized pore of parylene was prepared by photolithography, and then parylene was additionally deposited on to the micropore with the isotropic vaporization...
We describe a method for generating giant (micro sized) lipid vesicles using pneumatic valves and microfluidic T-junction. Water, oil containing lipids, and water were sequentially infused into the microfluidic channel to form bilayer lipid membranes (BLMs) on chambers (Fig. 1). Valves on the back of the chambers were driven pneumatically and pushed the water out of the chamber; consequently the BLMs...
This work presents a strategy that realizes efficient incorporation of membrane proteins in liposomes into a planar bilayer lipid membrane by using dielectrophoretic (DEP) force. The device consists of a chamber and a channel separated by a ??m-sized aperture that suspends a planar bilayer membrane. Applying an AC electric field across the membrane, the DEP force concentrates at the bilayer area therefore...
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