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Whole cells of Aspergillus niger CCM 8004 containing glucose oxidase (EC 1.1.3.4.) were used for the construction of an amperometric microbial mediated carbon paste biosensor. The microorganism was either placed on the surface of the electrode or incorporated directly into the carbon paste. The mediators were either dissolved in the buffer (hexacyanoferrate(III) and ferrocene) or loaded in the carbon...
Whole cells of Aspergillus niger strain CCM 8004 containing glucose oxidase (E.C. 1.1.3.4.) were used for the preparation of a glucose biosensor. The microorganism was entrapped in a dialysis membrane attached to the Clark oxygen electrode. The upper linearity limit of the sensor was improved by the presence of hydrogen peroxide. At a concentration of H 2 O 2 equal to 8 mmol 1 ...
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