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Glycobiology is dogged by the relative scarcity of synthetic, defined oligosaccharides. Enzyme‐catalysed glycosylation using glycoside hydrolases is feasible but is hampered by the innate hydrolytic activity of these enzymes. Protein engineering is useful to remedy this, but it usually requires prior structural knowledge of the target enzyme, and/or relies on extensive, time‐consuming screening and...
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