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The interaction of gold nanoparticles with Pfu DNA polymerase has been investigated by a number of biological, optical and electronic spectroscopic techniques. Polymerase chain reaction was performed to show gold nanoparticles' biological effect. Ultraviolet'visible and circular dichroism spectra analysis were applied to character the structure of Pfu DNA polymerase after conjugation with gold nanoparticles...
This work investigated the potential use of benzoic acid (BA)-based immunoadsorbent, a hydrophobic adsorbent, for autoantibodies selective adsorption therapy. The adsorbent was assessed through chromatographer and batch system assays in terms of adsorption capacity, selectivity for both polycloneantibodies in normal human plasma and pathogenic antibodies in patients' sera. For human immunoglobulin...
The E.coli expressed carrot antifreeze protein (DcAFP) was purified by preparative HPLC, refolded by dialysis, and checked by analytical HPLC and SDS-PAGE. The gradient-concentrations of DcAFP in 3% glycerol were used to cryopreserve rice single cell by vitrification at -25degC, -75degC and -180degC. The result showed that DcAFP, at optimal concentration of 0.1 mg/ml, can visibly inhibit the liquid...
Objective To evaluate the adsorbing effect of montmorillonite on uric acid and montmorillonite decreasing the level of uric acid in the serum. Methods The adsorbing effect of montmorillonite was observed in vitro and the effect of decreasing the serum concentration of uric acid of acute hyperuricemia model mice. The concentration of uric acid was measured by a method of uric acid enzyme and enzymatic...
Liposomes appear a promising approach as a drug-carrier system for topical application. The principal of this study was to investigate the influence of surfactants polyoxyethylene (40) stearate (S-40) and Tween 20 on the preparation and skin penetration of tea polyphenols (TP) liposomes. TP liposomes modified by surfactants were prepared by improved inverse-phase evaporation method. The particle size...
This paper reports the implementation and calibration of a microscopic three-electrode electrochemical sensor integrated with a polydimethylsiloxane (PDMS) microchannel to form a rapid prototype chip technology that is used to develop sensing modules for biomolecular signals. The microfluidic/microelectronic fabrication process yields identical, highly uniform, and geometrically well-defined microelectrodes...
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