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We made acute cardiac failure in excised cross-circulated canine hearts by a new coronary perfusion protocol consisting of Ca 2+ free Tyrode perfusion for the first 10 min, high Ca 2+ (16 mmol/l) Tyrode perfusion for the next 5 min, and normal Tyrode perfusion for the last 5 min interrupting blood cross circulation. After 50 min from the blood recirculation, left ventricular...
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