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Pyridoxal kinase catalyzes the ATP-dependent phosphorylation of vitamin B 6 , generating pyridoxal-5′-phosphate, an important cofactor for many enzymatic reactions. Pyridoxal kinase was purified 4300-fold to homogeneity from Trypanosoma brucei and peptides generated by proteolysis were subjected to amino acid sequence analysis. The peptide sequence information was used to generate a partial...
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