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The potency of 25‐hydroxyvitamin D3 (25(OH)D3) is increased by several fold through its metabolism into 1α,25‐dihydroxyvitamin D3 (1α,25(OH)2D3) by cytochrome P450 27B1 (CYP27B1). Thus, the pivotal role of 1α‐hydroxylation in the activation of vitamin D compounds is well known. Here, we examined the metabolism of 25‐hydroxy‐16‐ene‐23‐yne‐vitamin D3 (25(OH)‐16‐ene‐23‐yne‐D3), a synthetic analog of...
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