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We developed a gold coated glass chip bearing a poly(ethyleneglycol) (PEG) type compound as hydrophilic spacer for surface plasmon resonance studies, which enabled adequate estimation of K d value between FK506 and FKBP12 not only using purified FKBP12 (K d =22nM) but also using Escherichia coli lysate expressing FKBP12 (K d =15nM). These results indicated effectiveness of...
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