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Thermal stress of PSTVd-infected Nicotiana benthamiana led to appearance of a broad PSTVd sequence distribution, where most of mutations accumulated in the left half of the viroid's secondary structure including the ''pathogenicity'' domain. A similar effect had been reported for hop latent viroid [Virology 287 (2001) 349]. The pool of viroid ''thermomutants'' progenies was transcribed into cDNA and...
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