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An inulin fructotransferase (DFA III-producing) [EC 2.4.1.93] from Arthrobacter sp. L68-1 was purified and characterised. The enzyme was purified from culture supernatant of the microorganism 54.2-fold with a yield of 16.0% using DEAE-Toyopearl chromatography (repeated twice) and Super Q-Toyopearl chromatography. The enzyme showed maximum activity at pH 5.5–6.0 and 55°C. The enzyme activity was stable...
An inulin fructotransferase (DFA I-producing) [EC 2.4.1.200] from Arthrobacter pascens a62-1 was purified and the properties of the enzyme were investigated. The enzyme was purified from culture supernatant of the microorganism 58.5 fold with a yield of 8.32% using Super Q Toyopearl chromatography and butyl Toyopearl chromatography. It showed maximum activity at pH 5.5 and 45 o C and was stable...
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