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The mycobacterium smegmatis porin A (MspA) protein nanopore was prepared by E. coil prokaryotic expression system. The extraction of MspA nanopore using surfactants were carried out under the optimal conditions such as 0.5% extraction agent, 30 min of extraction and at 90 °C. The interactions of MspA nanopore with a variety of cyclodextrins were examined at single-molecule level. The interaction between...
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