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The sensitivity of current lateral flow strip (LFS) based assays is a technical bottleneck. The authors describe a method for signal amplification to give a 10- to 25-fold improvement of the detection limit without compromising the assay time and operational mode. The assays includes the following steps: (a) immobilization of melamine antibody on gold nanoparticles (AuNPs); (b) immobilization of BSA...
We have developed a lateral flow assay (LFA) for the detection of bisphenol A (BPA) in water samples. Antibody against BPA was labeled with gold nanoparticles, and these conjugates were used as the recognition probes for the construction of an LFA strip. The diameter of the gold nanoparticles, the amount of antibody, the pH of the buffer, and the categories of the conjugation pad were optimized. The...
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