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yqhD oxidoreductase was determined to be an NADP-dependent dehydrogenase, and was more active toward 3-HPA when compared to 1,3-propanediol oxidoreductase. To further improve enzyme activity towards 3-hydroxypropionaldehyde (3-HPA), error-prone PCR was implemented to mutant yqhD gene. Two mutants, D99QN147H and Q202A with increased catalytic and affinity efficiency, were obtained after one round of...
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