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Avian influenza virus (AIV) as a type of highly pathogenic influenza A virus, can not only cause serious illness and death in poultry but also threat human health and lead to public panic. Rapid, sensitive detection of AIV is urgent and significant for prevention and timely control of influenza epidemics. Herein, we reported an inductively coupled plasma mass spectrometry (ICP-MS) based method for...
A novel turn-on fluorescent probe, which coumarin-fused coumarin was used as fluorophore to link with 2, 4- dinitrodiphenyl ether moiety that has high selectivity and sensitivity for H2S and thiols, was designed and synthesized. Our probe YB can sensitively react with H2S and GSH to release free fluorophore with strong fluorescence signals. The probe YB could use as a significant molecular imaging...
In this work, ethylenediamine modified poly(glycidyl methacrylate-ethylene dimethacrylate)(poly(GMA-EDMA-NH2)) was prepared for highly selective extraction of Se(VI) with high adsorption capacity of 0.825 mg m−1 (530 µm i.d.). Based on it, a novel method of polymer monolithic capillary microextraction (CME) on-line coupling with inductively coupled plasma-mass spectrometry (ICP-MS) was developed for...
Prostate cancer (PCa) is a leading cause of cancer-related death among males globally. To date, prostate-specific antigen (PSA), as a typical tumour marker, has been widely used in the early diagnosis of PCa. However, in practical clinical tests, high serum levels of PSA show a high probability for false-positive results, leading to misdiagnoses. In this study, we developed a new classification system...
Arsenite (As(III)) has been considered as a human carcinogen associated with many human cancers especially skin cancer. Elucidation of the transformed species of As(III) during its metabolism in cells is beneficial for evaluation of its bioeffect. In this work, a hyphenated method of reversed phase ion pair high performance liquid chromatography - inductively coupled plasma mass spectrometry (RP-IP-HPLC-ICP-MS)...
A sensitive and selective TAPB-DMTP-COF (TAPB, 1,3,5-tris(4-aminophenyl)benzene; DMTP, 2,5-dimethoxyterephaldehyde; COF, covalent organic framework) modified carbon paste electrode was evaluated as a novel electrochemical sensor for the determination of lead in an aqueous medium. Lead was accumulated on the TAPB-DMTP-COF surface by the complexation with the amine groups, and detected by differential...
In this study,we proposed a triangular silver nanosheets (Tri-SNSs)-layered, Chitosan (CS)-supported three-dimensional of reduced graphene oxide (3D-ERGO) electrochemiluminescence (ECL) biosensing platform using self-designed dual-Ru(bpy)32+ scDNA (Ru2-DNA) as capture probe for ECL biosensing of single-chain DNA (scDNA). Based on the different affinity with scDNA and double chain DNA (dcDNA), the...
FexOy and ZrO2 nanoparticles co-doped layered porous polyimide, polyimide-FexOy-ZrO2 is prepared with a one-step strategy, shortly termed as PI-FexOy-ZrO2. The layered and porous structure of the polymer offers a supported platform for metallic oxide anchoring, exhibiting a mesopore size of 3.93 nm and providing a surface area of 198.47 m2 g−1. The metallic oxides were uniformly and highly dispersed...
A novel ternary hydrosulphonyl-based deep eutectic solvent (THS-DES) comprised of choline chloride/itaconic acid/3-mercaptopropionic acid (molar ratio 2:1:1) was firstly synthesized. The composition, property and microscopic structure of the new magnetic adsorbent (THS-DES@M-GO) based on the THS-DES modified the magnetic graphene oxide (M-GO) was characterized by the system. Magnetic solid-phase extraction...
Transferrin (TrF) is an important glycoprotein and disease biomarker that controls iron ion balance in the human body. Isolation and detection of TrF have important implication for the early detection of disease. Thus, a magnetic fluorescent molecularly imprinted nanoparticles (FMINPs) was prepared for extraction and fluorescence detection of TrF. The FMINPs was prepared with two steps, the first...
A novel label-free and enzyme-free detection strategy has been developed for the fluorescent detection of isocarbophos (ICP) using multi-walled carbon nanotubes (MWCNTs) and G-quadruplex as the signal transducers. In this work, the split ICP aptamer were attached to a G-quadruplex motif at their respective terminals. In the presence of ICP, the split aptamers could undergo conformational change into...
Lanthanide-doped nanoprobes have received significant attentions for utilization in biological sensing and imaging due to their unique optical properties. However, only few works were focused on fabrication of europium complexes based fluorescence polymeric nanoparticles (FPNs) with excellent biocompatibility and biodegradability. In this study, we fabricated the FPNs (named as Eu(TTA)3Phen-GluEG...
Metal oxide nanomaterials have been widely applied in the high-performance gas sensors. For metal oxide semiconductors, high surface-to-volume ratio and the exposed crystal facets are the two key factors for determining their gas sensing performances. In order to study the effect of surface structure on the gas sensing properties, in this work, two types of copper oxide (CuO) nanostructures, CuO nanotubes...
Nitrogen enriched carbon dots (CDs) are prepared by hydrothermal treatment with oxalic acid and ethylenediamine serving as raw materials. The obtained CDs display strong blue fluorescence emission at λex/λem = 320/410 nm, along with a quantum yield of 14%. It is interesting to obtain a linear increase of fluorescence with the variation of pH value within pH 5.01–8.94. Meanwhile, a favorable stability...
Methane-oxidizing bacteria (MOB), a unique group of Gram-negative bacteria utilizing methane as a sole source of carbon and energy, have been proved to be a biological indicator for gas prospecting. Field and cultivation-free detection of MOB is important but still challenging in current microbial prospecting of oil and gas (MPOG) system. Herein, SERS was used for the first time to our knowledge to...
Arsenic in hair and nails has been used to assess chronic exposure of humans to environmental arsenic. However, it remains to be seen whether it is appropriate to evaluate acute exposure to sub-lethal doses of arsenic typically used in therapeutics. In this study, hair, fingernail and toenail samples were collected from nine acute promyelocytic leukemia (APL) patients who were administered intravenously...
Nowadays, in order to improve the diagnostic accuracy of the disease, more and more contrast agents have been applied in the clinical imaging modalities. A combination of nanotechnology with optical, computed X-ray tomography (CT), and magnetic resonance imaging (MRI) has great potential to improve disease diagnosis and therapy. Herein, we developed a novel multimodal contrast agent for deep-tissue...
Simultaneous detection and differentiation of biomolecules is of significance in biological research. Biothiols such as cysteine (Cys), homocysteine (Hcy), and glutathione (GSH) play an important role in regulating the vital functions of living organisms. However, existing methods for simultaneous detection and differentiation of Cys, Hcy, and GSH are still challenging because of their similarity...
Exosomes have proved to be an effective cancer biomarker with significant potential, and several cell-specific molecules have been found in colorectal cancer (CRC) exosomes. Nevertheless, it is challenging to use exosomes in clinical lab diagnostics due to their nanoscale and the lack of a convenient and effective detection platform. Here, we developed a DNase I enzyme-aided fluorescence amplification...
In recent years, great concerns have been raised about salicylic acid (SA) and its derivatives as plant regulators. Therefore, precise determination of the distribution of SAs in the living plants is necessary for not only fundamental researches but also the regulating mechanisms. In this study, a custom-made solid phase microextraction (SPME) fiber based on diallyl dimethyl ammonium chloride-assembled...
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