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We demonstrate a simple new sensor design that exploits aptamer functionalized nanoparticles (NPs) to transduce the signal of aptamer receptors binding to target small molecules. An aptamer capable of binding to our target 17β-estradiol (E2) was isolated by SELEX with dissociation constant of 50nM and tethered to the surface of carboxylated polystyrene NPs. Upon exposing the aptamer functionalized...
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