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M13 is a filamentous Escherichia coli virus covered with five types of capsid proteins, in which pVIII with ~2700 copies was around the cylindered surface and pIII with five copies located at one end of the phage particle. The pIII-engineered M13 phages with enhanced binding specificity toward Fe were screened after five rounds of biopanning, and the one containing ATPTVAMSLSPL peptide at pIII-terminus...
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