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DIO3 and DIO3OS are two imprinted genes identified in mouse and humans. The DIO3 gene, which encodes for the type 3 deiodinase, is preferentially expressed from the paternal allele, while the DIO3OS transcript is transcribed in opposite orientation to DIO3, multiple noncoding and alternatively splicing isoforms from maternal allele. In this study, the five splice variants of DIO3OS were identified...
The INU1 gene encoding exo-inulinase cloned from Kluyveromyces marxianus CBS 6556 was ligated into the expression plasmid pINA1317 and expressed in the cells of the yeast Yarrowia lipolytica with high content of protein. The activity of the secreted inulinase was found to be 43.1±0.9U/ml after cell growth for 80h. When the engineered yeast cells were grown in the medium containing 4.0% inulin in 2-l...
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