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A silicon wafer with eight individually addressable microfabricated orifices was used for ion channel reconstitution and single-channel recording. A spin-on fluoropolymer created an insulating, hydrophobic interface that was more stable than silane. Total capacitance of the membranes was <10pF, making it easy to evaluate bilayer formation by capacitance change. Orifices of 50–250μm diameter were...
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