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Perhaps surprisingly, all electron microscopy (EM) data collected to date is less than a cubic millimeter – presenting a huge demand in the materials and biological sciences to image at greater speed and lower dosage, while maintaining resolution. Traditional EM imaging based on homogeneous raster scanning severely limits the volume of high-resolution data that can be collected, and presents a fundamental...
High angle annular dark field (HAADF)-scanning transmission electron microscope (STEM) data is increasingly being used in the physical sciences to research materials in 3D because it reduces the effects of Bragg diffraction seen in bright field TEM data. Typically, tomographic reconstructions are performed by directly applying either filtered back projection (FBP) or the simultaneous iterative reconstruction...
The use of transmission electron tomography for three dimensional morphology analysis of nanostructured materials has grown significantly in recent years due to the improved robustness of automated image collection, alignment and reconstruction routines. Although the amount of published 3D electron tomography data has increased accordingly, in many cases the data is used purely for visualization purposes,...
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