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Rice blast, caused by the ascomycete fungus Magnaporthe grisea, is one of the most serious diseases for cultivated rice. Sterol 14 alpha-demethylase (CYP51) is one of the key enzymes of sterol biosynthesis in biological kingdoms and an important drug target for microbial pathogenic infections. In the present study CYP51 with truncation of N-terminal residues from M. grisea (MGCYP51) was cloned and...
Streptococcus pneumoniae is the major causative organisms of meningitis, bacteremia, and pneumonia. The drug resistance of S.pneumoniae to antibiotics showed ascending tendency. The present study cloned the full-length HMG-CoA reductase (HMGR) gene of S.pneumoniae and expressed in E.coli BL21 (DE3). The His-tag containing recombinant protein was purified by Ni-NTA affinity chromatography. Statins...
Methyl parathion hydrolase mph gene was cloned from a novel bacterium Pseudomonas stutzeri HS-D36. The phylogenetic tree was constructed and the structure was predicted by bioinformatics. Results showed MPH protein was 99.0% similar to MPD protein (from pseudomonas sp. WBC-3) and it belonged to metallo-beta-lactamases family. There is an alpha/beta barrel structure in MPH structure. Two independent...
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