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We synthesized a new botulinum neurotoxin type A (BoNT/A) sensing material consisting of a photopolymerizable polyacrylamide hydrogel incorporating a 25-mer BoNT/A recognizable peptide substrate coupled to an acryloyl-PEG-NHS crosslinker that readily reacts with free amines at pH 8–9 via the NHS moiety. The peptide substrate residues are slightly modified from synaptosomal-associated protein SNAP-25...
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